Czech Republic: A Rising Hub for Innovation
Along with Estonia and Poland, which are established players in the CEE startup scene, the Czech Republic demonstrates a strong ambition to become a hub for revolutionary tech ideas. Moreover, the startup Czech Republic ecosystem is currently in 3rd position in the regional rating, while the country successfully reached 32nd place worldwide. These impressive results stem from the Czech’s unique potential to develop scalable and high-quality products and services. Therefore, 2025 is a definite year for discovering promising ventures, and the Czech startup scene is a decent destination for entrepreneurs to pursue their business goals. The following reading will introduce startups in Czech Republic that are worth mentioning due to their original products and stable performance.
A Brief Insight into the Czech Startup Ecosystem
The Czech Republic is home to Avast, GoodData, and Rohlik Group which solidified its international reputation with its commitment to quality and innovation. In this context, the Czech startup ecosystem represents a vibrant and diverse business environment, which encompasses favorable regulatory and economic conditions for newcomers. The Czech Republic built its legacy through consistent improvement of the educational system, and 50, 000 students graduate from prestigious institutions annually, thereby shaping a new generation of engineers and IT professionals. In addition to a skilled workforce, political stability, and taxation, the featured country offers a developed infrastructure with its strategic allocation for new startups. For instance, Brno has become a leading high-tech hub in the Czech Republic, while this city is regarded as Czech Silicon Valley due to the investments in R&D and educational segments.
Czech Startups in 2025: Embracing Innovation and Growth
The highly competitive environment also stimulates the growth of the Czech startup scene, where entrepreneurs must be inclined toward disruptive tech trends to seize their market share. Moreover, the Czech startups news highlights the discussed peculiarities of the ecosystem, implying the unique opportunities of capitalizing on businesses. 2025 will be a crucial time for companies to take advantage of next-gen AI technologies. The listed Czech startups have already presented their tailored solutions to customers, securing leading positions in the industry. Thus, the picked Czech-based companies offer unique value to the market while exhibiting steady financial growth.
Rohlik
This brand is a definition of resilience, while its ability to create relevant products redefines the food retail industry. Without a doubt, Rohlik deserves its attention in Czech startups news as this company shapes a new vision for e-grocery by standing at the forefront of its revolutionary digitalization. The unprecedented tech solutions and seamless infrastructure set a new industry blueprint for the supply chain and service delivery process.
Woltair
The next pick is a fresh startup that focuses on developing customized renewable energy solutions. In the current era of sustainable growth and development, Woltair is one of the best Czech startups known for its visionary mindset and ability to foresee economic trends. Woltair’s comprehensive approach encompasses sales, installation, and maintenance services, ensuring customers receive end-to-end support for their renewable energy needs. Powered by the latest technological innovations, Woltair makes eco-friendly heating systems and clean energy sources accessible to industries.
Upvest
Upvest retains its solid reputation as a leading investment platform specializing in real estate private debt and equity. In regard to the startup Czech Republic ecosystem, this project represents a successful example of digitalization of the designated market by integrating tech solutions that enhance transparency and accessibility of services. Indeed, this venture holds its deserved position for making a shift in traditional real estate practices.
Keboola
As modern businesses look for SaaS products designed for data management, Keboola revitalizes the industries by offering its impressive variety of services. The chosen brand is one of the promising AI startups that will make a difference in the Czech startup scene in 2025. From automation to the deployment of customized AI platforms, Keboola offers comprehensive solutions to enterprises that strive for operational efficiency and shaping their competitive edge.
Eyeball
This brand stands out from the list by offering coaching and youth football scouting that embrace advanced digital technologies. Eyeball is an inspiring digital service, which implements the newest data analytics, making it a truly pioneering project among popular Czech startups. Moreover, this original tech solution to talent sourcing has a high potential to become a big name in the sports industry.
Birdwingo
Birdwingo complements the list of 25 notable startups from the Czech Republic by offering its edtech product. Additionally, this brand has a leading position among available educational startups in Czech Republic. The user-friendly and interactive format of learning allows its users to enhance their financial literacy, while Birdwingo seeks the opportunity to collaborate with educational institutions to fulfill its mission.
Sotio
Sotio is one of the influential startups from Prague that aims to develop innovative cancer immunotherapies. This pick exemplifies how entrepreneurs can invest in startups to make a meaningful impact on people’s lives and health through innovation and collaboration with emerging biotech firms. It is reasonable to regard this company as a progressive startup, which will offer its distinguishing contribution to the development of cutting-edge cancer therapies.
Better Stack
This enterprise is a Prague-based developer of a downtime management platform, allowing its customers to manage any technology stack or debug issues. In turn, Better Stack is a decent example of the many competitive tech startups from the Czech Republic. The brand’s products encompass a modern vision of engineering while preserving simplicity in the service delivery process.
Readmio
This startup reinvents early childhood learning with a blend of technology and storytelling. In the context of original ideas, Readmio is definitely one of the best Czech startups under this category. A new interactive mode of storytelling with the accessibility of the product proves that tech innovations have an unlimited potential to create exceptional value for people. Concerning Readmio’s features, the application synchronizes audio elements with the narrative when users read aloud the text, improving engagement with the story.
VOS.health
HR managers understand how burnout and mental health affect productivity, while declining well-being substantially increases the risks of employee turnover. VOS.health offers an original and relevant solution by offering comprehensive digital solutions to businesses, granting its deserved spot among the top Czech startups in 2025. A universal SaaS product offers personalized well-being plans, self-care exercises, and AI-powered features to improve people’s health. While VOS.health is designated for business, this application can be a decent choice for managing personal health or community. This brand demonstrates how startups can make a meaningful impact, thereby raising the topics of people’s health and happiness.
Superface
Superface empowers developers to build more capable and versatile AI applications that improve their ability to interact with a wide range of external services and tools. When discussing the promising AI startups in 2025, this pick stands out with seamless data exchange and enhancing the functionality of AI-driven applications provided by its tech solutions. Superface offers a definitive set of features, including universal compatibility with major AI frameworks, instant integration with popular tools like Salesforce and Slack, intelligent tooling that ensures seamless function calls, serverless compatibility for flexible deployment, and managed authentication to simplify secure API access. In regard to highly performing startups, this brand provides an incredibly powerful platform for developers building AI-driven applications that ensures its competitiveness.
Hedepy
Hedepy is another unique example of healthtech services powered by innovation. Startups in Czech Republic encompass a unique ability to foresee emerging trends among major industries, especially healthcare, while developing suitable products to meet the demands of customers. In turn, this startup leverages digital technology to provide online therapy and mental health support by connecting individuals with certified therapists through an accessible, affordable, and user-friendly platform. This service also emphasizes the crucial role of a client-centered approach to treatment while upholding the strict ethical frameworks to ensure the best outcomes.
Twisto
Twisto represents a next-gen fintech service that makes financial management and payments look effortless. As one of the promising tech startups, this brand offers convenient and user-friendly services based on subscription plans. Financial flexibility and control become a top priority for consumers, while the featured product makes everyday finances more manageable for a reasonable price.
Lakmoos
The current merge of behavioral science with AI causes a paradigm shift in market research. Lakmoos represents how AI startups can become a part of the transformational process by offering their compelling services. Specifically, its AI-driven analytics with forecasts introduces a modern approach to market research, delivering timely and accurate insights. This company empowers businesses to understand and anticipate consumer behavior, thereby correcting strategic decision-making and planning in real-time.
CRUXO
The future of e-commerce is predetermined by the increasing role of automation and big data, where entrepreneurs must find and implement a suitable tech solution. Cruxo represents innovative Czech Republic startups by offering a data-driven platform designed to streamline and enhance retail media operations. This brand empowers companies to boost revenue, improve advertising relevance, and save time through its user-friendly interface of the SaaS platform.
Klaro
The chosen brand is a unique startup and a trusted partner for healthcare facilities alsoseeking reliable and efficient equipment solutions. This pick may contrast with the rest of the tech startups from the list, though Klaro has become a significant supplier of handling carts in the Czech market, known for its commitment to quality and innovation. Moreover, the full customization of products for a reasonable price is a crucial feature of this brand, making it a noteworthy startup.
Resistant AI
While AI technologies foster innovation and creativity, the same trend poses serious threats to data safety and protection. In turn, Resistant AI represents a leading brand of Czech AI startups that develop comprehensive solutions for data security and fraud prevention. Powered by machine learning, the company’s AI solutions include document, identity, and transaction forensics aimed at minimizing fraud risks. 2025 will be a year of fundamental transformation among industries, while fincrime will also become a new problem for companies as criminals will attempt to use AI for their purposes. Resistant AI embraces a new approach to data protection by addressing these threats while utilizing machine learning to combat these issues.
Memsource Group / Phrase
Phrase is a visionary product that embodies a new generation of entrepreneurs where culture has a decisive role in brand value. According to its mission statement, this company strives to surpass language barriers by offering translation and localization through advanced AI-powered solutions, making it one of the most inspiring Czech Republic startups on the list. This cloud-based translation platform encompasses the future of business, where a commitment to high-quality, scalable services meets diversity, transparency, and security to maximize customer satisfaction.
LIV
Entertainment comprises a considerable part of modern life, which depends on the emergence of innovative technologies. Entrepreneurs further invest in startups like LIV to seize their opportunity to launch an impactful trend in the industry. The chosen startup is a VR streaming platform, and it is possible to regard this service as an advanced mixed-reality and VTubing solution for VR content creators. VR technology can become one of the trending products in 2025, and LIV can seize the opportunity to gain its attention beyond the niche market.
Time is Ltd.
Modern entrepreneurship is based on collaboration, where professionals exchange their experience and creative ideas to develop a unique product. In this context, entrepreneurs invest in startups that can improve employee engagement, collaboration, and productivity to meet this demand. Time is Ltd. is a promising Czech startup, which enables data-driven decision-making to optimize workflows and reduce costs for its customers. Additionally, this project further offers tailored solutions for HR, sales, and managerial departments, boosting overall productivity.
Tropic Square
Developing reliable and highly functional hardware can be a daunting task, while large companies prioritize comprehensive security measures, adding new complexities for manufacturers. Czech Republic startups do not fail to provide original solutions in different industries, and Tropic Square redefines hardware safety through a unique engineering mindset by incorporating Kerckhoffs’ principle. Therefore, this principle allows this startup to develop custom solutions, catering to the latest requirements of high-security hardware. This vision provides a chance to become one of the pioneering companies that produce secure element chips based on open architecture.
Flowpay
This startup is a convenient and simple fintech solution for its customers, providing all essential tools to manage and plan financial expenditures. Czech tech startups should regard this promising company for its fresh perspective on the service delivery process. Minimization of bureaucratic impact with advanced analytical tools comprises one of the main features of Flowpay, which denotes a critical value for enterprises and customers in 2025.
Daktela
SaaS solutions gradually substitute traditional programs due to their cost-effectiveness and portability. Daktela strengthens its market position among Czech startups by embracing this transformation. The SaaS communication platform designed for contact centers embeds customizable, cloud-based, and scalable characteristics, making Daktela’s services suitable for businesses of all sizes. As communication will become an integral part of the commercial success of any brand, Daktela has a unique chance to make its breakthrough in the industry.
Choice
This article reveals how the featured companies encompass disruptive technologies with an original vision of future entrepreneurship. Choice is one of the prominent startups in Czech Republic that redefines service delivery processes for restaurants. Their platform integrates with POS systems, offers CRM capabilities, and supports loyalty programs, helping restaurants to digitalize their fundamental operations. It is a cost-effective and fresh approach to managing restaurant businesses, and Choice has embraced a new trend in the industry, shaping a distinguishing brand image.
Supernova.io
This company closes the list of top Czech startups, while it provides one of the comprehensive platforms for managing, automating, and scaling design systems. Concerning its current commercial success, Supernova is a decent example of why entrepreneurs should invest in startups like the featured one. Thus, this startup is a perfect choice for organizations aiming to streamline their design-to-code processes and enhance efficiency.
The Road Ahead for the Czech Startup Scene
In conclusion, 2025 will be a promising year for startups, where the featured companies have equal chances to continue their expansion. Czech startups news will eventually mention some of these brands in the observable future, which is inevitable due to their ability to embrace emerging trends. Moreover, investors and entrepreneurs can learn from the shared experiences provided by startups from this list. These startups share the familiar commitment to redefining traditional services or products, thereby setting a new standard for industries. This inclination towards quality and value is a driving force of the Czech startup scene, which will strengthen the country’s leadership in the CEE region. The culture of innovation and patient-centered mindset further exemplify why the Czech Republic retains its influence on the European economy and beyond.
